Part:BBa_K678000:Design

DMKP-P6, Aspergillus nidulans promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 23
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 23
  Illegal NheI site found at 473
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 23
  Illegal XhoI site found at 122
  Illegal XhoI site found at 132
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 23
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 23
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 212
  Illegal BsaI.rc site found at 499
  

Design Notes

This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.

This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.

This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmid pJEJAM18

Source

Aspergillus nidulans genomic DNA (AN1122)

References

Dorte Koefoed Holm, Center for Microbial Biotechnology, Technical University of Denmark (Unpublished data).